A new restriction endonuclease from Bacteroides distasonis (BdiI).
نویسندگان
چکیده
منابع مشابه
Characterization of restriction endonuclease BdiSI, an isoschizomer of SfeI, from Bacteroides distasonis strain S-7.
weight), cultivated anaerobically at 37°C for 24hr in a medium containing 0.4% casamino acids, 0.3% yeast extract, 0.3% peptone, 1.0% glucose, 0.15% sodium chloride, 0.1% Tween 80 and 0.04% L-cysteine, pH 7.2, were disrupted by passing through a French pressure cell (Ohtake Works Co.) at 1500-1700kg/cm2. Nucleic acids were removed from the cell extracts by 1%streptomycin treatment, and then the...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1990
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.54.275